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Marek’s sampling

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  • RAHUL LANJE

    Member
    July 31, 2025 at 6:05 pm

    Nice information

  • Lasisi Samuel Olakunle

    Member
    July 8, 2025 at 3:03 pm

    educative… Thank you..

  • Amir Sohel

    Member
    July 5, 2025 at 2:15 am

    Well appreciated

  • Pragati Salutgi

    Member
    July 2, 2025 at 8:08 am

    👍👍

  • Muhammad Zeeshan Asghar

    Member
    July 1, 2025 at 11:21 am

    For the detection of Marek’s Disease virus (MDV), suitable samples and recommended sizes are as follows:

    Suitable Samples

    1. Tissues:

      • Lymphoid Tissues: Spleen, liver, and bursa of Fabricius are ideal for viral detection.
      • Nerve Tissue: Sciatic nerve or other peripheral nerves can also be sampled.

    2. Blood:

      • Whole Blood or Serum: Blood samples can be used for serological testing.

    3. Feather Follicles:

      • Feather Tips: Samples of feather follicles can be collected, especially in live birds.

    4. Swabs:

      • Tracheal Swabs: Useful for detecting the virus in respiratory secretions.

    Recommended Sample Size

    • Tissue Samples: A sample size of approximately 1-2 grams from each tissue type is usually sufficient for PCR or virus isolation.
    • Blood Samples: A minimum of 1-2 mL of blood is typically required for serological tests.
    • Feather Follicles: Collecting 5-10 feather tips can provide adequate material for testing.
    • Swabs: Use standard swabs, ensuring to sample thoroughly from the target area.

    Conclusion

    Collecting a variety of samples, including lymphoid and nerve tissues, blood, feather follicles, and swabs, with the recommended sizes, enhances the chances of accurately detecting Marek’s Disease virus.

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