mycoplasma

[Saviour Uyinosa]

Serology:

• Detects antibodies (IgM or IgG) against Mycoplasma in blood serum.

• Common tests include enzyme-linked immunosorbent assay (ELISA) and complement fixation.

• Useful for confirming past or current infections, though it may not distinguish active from resolved cases without paired samples (acute and convalescent).

[Saviour Uyinosa]

Polymerase Chain Reaction (PCR):

• PCR is a rapid, sensitive, and widely used molecular tool to detect Mycoplasma DNA.

• Targets specific genes (e.g., 16S rRNA) in samples like respiratory secretions, blood, or tissue

[Saviour Uyinosa]

Culture-Based Methods:

• Mycoplasmas are fastidious organisms requiring special growth media (e.g., PPLO broth or agar with supplements like horse serum).

• Samples (throat swabs, sputum, or tissue) are cultured under specific conditions (e.g., 37°C with 5% CO₂).

• This method is slow (days to weeks) and less commonly used due to the difficulty of growing Mycoplasma.

[Dr Yamna Ahmad]

Common diagnostic tools for Mycoplasmosis in poultry

Serological test

1.Rapid Serum Agglutination (RSA)

2.ELISA (Enzyme-Linked Immunosorbent Assay)

3.Hemagglutination Inhibition (HI) Test

Molecular and Cultural Based Test

1.PCR

2.Culture Isolation Test

[Amir Sohel]

Common diagnostic tools for mycoplasmosis include culture, serological tests (looking for antibodies), and molecular tests like PCR (detecting the bacteria’s DNA).

[Olayiwola Danso]

1. Clinical signs and post mortem examination

2. Serological tests.

3. Molecular tests (DNA Detection)

4. Bacterial culture and isolation

5. Histopathology (Microscopic examination).

[Md. Osman Sheikh]

<mark>Common diagnostic tools for mycoplasmosis include culture, serology, and molecular methods like PCR.</mark>